Methods of Assisted Reproduction May Be Connected to Risk of Using Sperm with Impaired DNA Integrity

That Can Lead to Spontaneous Abortion or Delivery of a Child With Congenital Abnormalities

Przegl Lek. 2006;63(9):800-2.Links
[Influence of DNA damage on fertilizing capacity of spermatozoa][Article in Polish]


Sikora J, Kempisty B, Jedrzejczak P, Jagodziński PP.
Katedra i Zakład Biochemii i Biologii Molekularnej Akademii Medycznej im. K. Marcinkowskiego w Poznaniu.

DNA damage in the male germ line cells is correlated with male infertility. Nuclear DNA damage in mature sperm includes single strand nicks and double strand breaks that can arise as a consequence of errors in chromatin rearrangement during spermiogenesis, abortive apoptosis, and oxidative stress. Methods of assisted reproduction may be connected with a risk of the using of sperm with impaired DNA integrity that can lead to spontaneous abortion or delivery of a child with congenital abnormalities.

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1: Asian J Androl. 2006 Jan;8(1):11-29. Links
Sperm chromatin structure and male fertility: biological and clinical aspects.Erenpreiss J, Spano M, Erenpreisa J, Bungum M, Giwercman A.
University of Lund, Fertility Centre, Malmö University Hospital, Malmö SE 205 02, Sweden. Juris.Erenpreiss@med.lu.se

Sperm chromatin/DNA integrity is essential for the accurate transmission of paternal genetic information, and normal sperm chromatin structure is important for sperm fertilizing ability. The routine examination of semen, which includes sperm concentration, motility and morphology, does not identify defects in sperm chromatin structure. The origin of sperm DNA damage and a variety of methods for its assessment are described. Evaluation of sperm DNA damage appears to be a useful tool for assessing male fertility potential both in vivo and in vitro. The possible impact of sperm DNA defects on the offspring is also discussed.

PMID: 16372115 [PubMed - indexed for MEDLINE]


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Genomics. 2002 Jan;79(1):58-62. Links
Paternal origins of complete hydatidiform moles proven by whole genome single-nucleotide polymorphism haplotyping.Fan JB, Surti U, Taillon-Miller P, Hsie L, Kennedy GC, Hoffner L, Ryder T, Mutch DG, Kwok PY.
Affymetrix, 3380 Central Expressway, Santa Clara, CA 95051, USA.

Complete hydatidiform moles (CHMs) are diploid tumors that result from fertilization of an empty ovum by a haploid 23,X sperm. In most cases, the resulting duplication of the genome gives rise to a 46,XX genotype and is thought to be androgenetic in origin. If this hypothesis is correct, then the genotypes of all polymorphic markers in CHMs should be homozygous. We used a dense set of single-nucleotide polymorphism (SNP) markers, evenly spaced throughout the genome, to definitively test this hypothesis. We genotyped genomic DNA samples from five CHMs and their corresponding maternal samples with 1494 SNP markers using high-density microarrays (HuSNP). As predicted, the maternal samples were heterozygous at >25% of the markers, which is consistent with the expected average heterozygosity of this panel of SNPs. In contrast, the five CHM samples were heterozygous at <0.75% of the SNP markers, which shows that these diploid tumors consist of a duplicated set of chromosomes. Because the CHM genotypes represent the haplotypes of their genomes, our results show that long-range haplotypes can be obtained easily with this resource and that a collection of such samples is a simple way to obtain reference haplotypes for association studies in various populations.

PMID: 11827458 [PubMed - indexed for MEDLINE]